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What is the main purpose of sample grinding in DNA isolation?

Answer and explanation

Correct answer: Mechanically disrupting tissue and cells to improve access of lysis buffer

Grinding breaks the tissue into smaller pieces and disrupts cells mechanically. This increases the contact of lysis buffer and enzymes with the cells, making it easier to release DNA. Option B is incorrect because DNA isolation aims to release DNA from cells, not keep it stable inside them. Exam tip: associate grinding with tissue disruption before cell lysis.

Related tags

Dna IsolationSample GrindingTissue DisruptionCell LysisRecombinant Dna TechnologyIsolation Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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