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In bacterial DNA isolation lysozyme acts on which target?

Answer and explanation

Correct answer: Peptidoglycan cell wall

Lysozyme hydrolyses the β-1,4 glycosidic bonds in peptidoglycan of the bacterial cell wall. This weakens the wall and makes cell lysis easier for releasing DNA. Phospholipids of the cell membrane are generally disrupted by detergents, not by lysozyme. Exam tip: associate lysozyme in bacterial DNA isolation with the peptidoglycan cell wall.

Related tags

LysozymePeptidoglycanBacterial Dna IsolationCell LysisRecombinant Dna TechnologyIsolation Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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