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Which structures must be disrupted in animal-tissue DNA isolation?

Answer and explanation

Correct answer: The cell membrane and nuclear membrane

Animal cells do not possess a rigid cell wall or chloroplasts, but their plasma membrane encloses the cytoplasm and a nuclear envelope encloses the DNA-containing nucleus. Efficient isolation therefore requires disruption of the cellular membrane and access to the nucleus; lysis conditions and detergents help solubilize lipid membranes. Additional treatment may remove proteins associated with DNA, but that does not change the basic structural requirement. Option C is correct because both barriers must be opened for nuclear DNA to be released. Option A is incomplete, while soil layers and chloroplast walls are not normal structures of animal tissue and cannot explain animal DNA extraction.

Related tags

Animal TissueCell MembraneNuclear MembraneIsolation Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq

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