What is the practical workflow of DNA isolation?DNA isolation का practical workflow कौन-सा है?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: Sample lysis, cleanup, precipitation, quality check, and storageSample lysis, cleanup, precipitation, quality check, and storage
A practical DNA-isolation workflow begins with collecting and identifying the sample, followed by lysis to disrupt cells and release nucleic acids. The lysate is then cleared and cleaned so proteins, lipids, cell debris, and inhibitory chemicals are removed. DNA may subsequently be precipitated or bound to and eluted from a purification matrix, depending on the protocol. The preparation is checked for concentration, purity, and sometimes integrity before being stored under suitable conditions for later use. Option A gives this logical sequence. The other choices list unrelated biological processes or omit the essential recovery and quality-control steps required to obtain usable DNA.DNA isolation का practical workflow sample को collect और identify करने से शुरू होता है। इसके बाद lysis द्वारा cells को तोड़कर nucleic acids बाहर निकाले जाते हैं। Lysate को clear और clean करके proteins, lipids, cell debris तथा inhibitory chemicals हटाए जाते हैं। Protocol के अनुसार DNA को precipitate किया जा सकता है या purification matrix पर bind करके elute किया जा सकता है। फिर concentration, purity और कभी-कभी integrity की जाँच की जाती है तथा DNA को उचित conditions में store किया जाता है। इसलिए विकल्प A सही है। बाकी विकल्प असंबंधित biological processes बताते हैं या DNA recovery और quality control जैसे आवश्यक चरणों को छोड़ देते हैं।
Student feedback
Was this question useful?
👍 0 Helpful 👎 0 Not helpful
Yes 0% No 0%
0 responsesStudent Reviews
No published reviews yet.
