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What can good DNA purity but low yield mean?

Answer and explanation

Correct answer: DNA is clean but its amount is low

DNA purity and DNA yield describe two different properties of an extracted sample. Purity indicates how much the preparation is free from substances such as proteins, salts, phenol, or other nucleic acids, whereas yield indicates the total quantity of DNA recovered. Therefore, a sample can have a satisfactory absorbance ratio and still contain only a small amount of DNA because the starting material was limited, cell lysis was incomplete, or DNA was lost during transfer, washing, or elution. Option A is correct. Low yield alone does not prove contamination, and it certainly does not mean that DNA became protein or that no DNA is present.

Related tags

Dna PurityDna YieldQuality AssessmentIsolation Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq

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