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What can be one reason for low DNA yield?

Answer and explanation

Correct answer: Incomplete cell lysis

DNA can be recovered only after it is released from the cells during lysis. If lysis is incomplete, a significant amount of DNA remains trapped inside the cells, resulting in a low DNA yield. Complete lysis and efficient DNA precipitation generally improve yield rather than reduce it. Exam tip: For low-yield questions in DNA isolation, check errors in lysis, DNA degradation, and precipitation steps.

Related tags

Dna IsolationLow Dna YieldCell LysisRecombinant Dna TechnologyDna ExtractionIsolation Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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