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What can be the role of chloroform in DNA isolation?

Answer and explanation

Correct answer: Helping phase separation and protein removal

Chloroform is an organic solvent used during extraction-based DNA isolation. It does not create new DNA, copy DNA, make RNA, or dissolve agar. Its important practical role is to help separate the mixture into different liquid phases. Proteins and other unwanted cell materials can move into the organic phase or collect at the boundary, while DNA is retained in the suitable aqueous phase under the proper conditions.

Thus, option A is correct because chloroform supports phase separation and helps remove proteins and other impurities. The exact separation also depends on the complete extraction mixture and careful centrifugation. Option B describes amplification, which requires enzymes and nucleotides, while option C is transcription and option D is unrelated to chloroform’s extraction function. The supplied answer and explanation are scientifically consistent.

Related tags

ChloroformPhase SeparationProtein Removal

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