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Why can polysaccharides be a problem in plant DNA isolation?

Answer and explanation

Correct answer: They can make the solution viscous and affect DNA purity and enzyme reactions

During plant DNA isolation, polysaccharides may co-precipitate with DNA. This can make the sample viscous, reduce DNA purity, and interfere with enzyme-based reactions such as PCR or restriction digestion. Option C describes the action of restriction enzymes, not polysaccharides. Exam tip: The CTAB method is especially useful for removing polysaccharide contaminants from plant DNA preparations.

Related tags

Plant Dna IsolationPolysaccharidesCtab MethodDna PurityEnzyme InhibitionIsolation Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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