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What problem can occur with an old or poorly stored sample?

Answer and explanation

Correct answer: DNA quality may decrease

The governing concept is nucleic-acid stability during storage. An old or poorly stored biological sample may experience repeated warming, moisture exposure, unsuitable pH, microbial activity, or action by nucleases. These factors can fragment DNA or chemically damage it, lowering its integrity and sometimes reducing the recoverable yield. Hence option A is correct. Degraded DNA may appear as a smear rather than a sharp high-molecular-weight band and may perform poorly in long-amplicon PCR or other applications. Option B is false because storage does not guarantee purity. Option C is unrelated, since proteins may remain or degrade independently. Option D is also false because lysis is still required to release DNA from cells.

Related tags

Old SampleDna DegradationStorage StabilityIsolation Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq

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