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How can intact genomic DNA appear on agarose gel?

Answer and explanation

Correct answer: As a high-molecular-weight band near the well

Intact genomic DNA is very large, so it migrates slowly through agarose gel and usually appears as a high-molecular-weight band near the well. In contrast, broken or degraded DNA contains fragments of many sizes and commonly produces a smear in the lane. Exam tip: a distinct heavy band near the well suggests intact DNA, whereas a smear suggests degradation.

Related tags

Genomic DnaAgarose GelDna IsolationDna IntegrityElectrophoresisRecombinant Dna TechnologyIsolation Of DnaProcesses Of Recombinant Dna Technology

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