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Why is it important to inactivate nucleases in DNA isolation?

Answer and explanation

Correct answer: To prevent DNA degradation

Nucleases are enzymes that can cut phosphodiester bonds in DNA and break it into smaller fragments. Inactivating them during DNA isolation protects the extracted DNA and keeps it intact, so option D is correct. Removing salts is part of purification, not the purpose of nuclease inactivation. Exam tip: DNases are nucleases that specifically degrade DNA.

Related tags

Dna IsolationNucleasesDna DegradationDnaseRecombinant Dna TechnologyIsolation Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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