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What is the next main goal after cell lysis in DNA extraction?

Answer and explanation

Correct answer: Removing impurities from DNA

The governing concept is sequential DNA purification. Cell lysis releases DNA together with proteins, membrane lipids, RNA, salts, and other cellular components. The next main objective is therefore to separate these contaminants while keeping the DNA in solution or precipitating it in a controlled way. Option A is correct because removal of impurities improves DNA purity and makes the sample suitable for PCR, restriction digestion, electrophoresis, or other analyses. Options B, C, and D describe transformations that do not occur during extraction and have no laboratory purpose in this step. Depending on the protocol, impurity removal may involve detergents, proteases, organic extraction, binding to silica, washing, or selective precipitation.

Related tags

Post-LysisDna PurificationImpurity RemovalIsolation Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq

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