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Why does keeping samples cold help in DNA isolation?

Answer and explanation

Correct answer: The activity of nucleases that break down DNA slows down

During DNA isolation, nucleases released from cells can cut and degrade DNA. Low temperature slows the activity of these enzymes, helping to preserve the DNA. Increased DNA replication is not the reason, because DNA is not replicated during the isolation procedure. Exam tip: Cold conditions and nuclease inhibitors are used to protect DNA from degradation.

Related tags

Dna IsolationNuclease ActivityCold TemperatureDna DegradationRecombinant Dna TechnologyIsolation Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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