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Why is EDTA present in TE buffer?

Answer and explanation

Correct answer: To protect DNA from nucleases

The governing concept is long-term protection of stored DNA. TE buffer contains Tris to help maintain a suitable pH and EDTA to chelate divalent metal ions such as magnesium. Nucleases often need these ions for activity; by binding them, EDTA reduces nuclease-mediated degradation and helps preserve DNA during storage. Thus option D is correct. EDTA does not directly amplify DNA, produce ethanol, or construct a membrane. Amplification requires a separate reaction containing template, primers, nucleotides, polymerase, and suitable conditions. The protective function of EDTA explains why it is included in TE buffer even though it is not itself a DNA-amplifying reagent.

Related tags

EdtaTe BufferNuclease ProtectionIsolation Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq

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