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Why can DNase enzyme be harmful for a DNA sample?

Answer and explanation

Correct answer: It can break DNA into smaller fragments.

DNase is a nuclease enzyme that can cleave phosphodiester bonds in DNA, degrading it into smaller fragments. Therefore, its presence during DNA isolation can reduce both the quantity and quality of the DNA sample. DNase does not convert DNA into protein. Exam tip: Chelating agents such as EDTA are used during DNA isolation to inhibit DNase activity.

Related tags

DnaseDna DegradationDna IsolationNuclease EnzymesRecombinant Dna TechnologyIsolation Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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