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Class 11BiotechnologyProcesses of Recombinant DNA TechnologyIsolation of DNAEasy
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What is the main role of detergent in DNA isolation?

Answer and explanation

Correct answer: Dissolving lipids of cell and nuclear membranes

Detergent dissolves the lipids of the cell membrane and nuclear membrane, causing membrane lysis and releasing DNA into the solution. It does not cut DNA into fragments; restriction enzymes perform that function. Exam tip: associate detergent in DNA isolation with membrane lysis.

Related tags

Dna IsolationDetergentMembrane LysisCell MembraneNuclear MembraneRecombinant Dna TechnologyIsolation Of DnaProcesses Of Recombinant Dna Technology
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10 more MCQs
  1. 1What is the main purpose of DNA isolation?
  2. 2What is a common first task in DNA isolation?
  3. 3Why may breaking the cell wall be necessary in DNA isolation?
  4. 4What is the process of breaking the cell membrane called?
  5. 5Detergents like SDS are linked with what in DNA isolation?
  6. 6Why are enzymes like protease useful in DNA isolation?
  7. 7Why is RNase used in DNA isolation?
  8. 8What is the main role of EDTA in DNA isolation?
  9. 9Why can DNase enzyme be harmful for a DNA sample?
  10. 10What is one role of salt in DNA isolation?
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