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Why may breaking the cell wall be necessary in DNA isolation?

Answer and explanation

Correct answer: To release DNA from inside the cell

The cell wall is a rigid outer layer, especially in plant, fungal, and bacterial cells. DNA is present inside the cell, so the cell wall and then the cell membrane must be disrupted to release the cellular contents containing DNA. Staining DNA may be used later for analysis, but it is not the purpose of breaking the cell wall. Exam tip: In DNA isolation, cell-wall disruption is done for cell lysis and release of DNA.

Related tags

Dna IsolationCell WallCell LysisRecombinant Dna TechnologyBiotechnologyIsolation Of DnaProcesses Of Recombinant Dna TechnologyClass 11 Mcq

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