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When can exonuclease treatment be useful in restriction digestion work?

Answer and explanation

Correct answer: In processing specific unwanted DNA ends or single-stranded regions

An exonuclease removes nucleotides from free DNA ends. Thus, after restriction digestion it can be used to process unwanted DNA ends or, in suitable cases, single-stranded overhangs. DNA visualization requires stains such as ethidium bromide or other dyes, not an exonuclease. Exam tip: exonucleases act from DNA ends, whereas endonucleases cut at specific sites within a DNA strand.

Related tags

ExonucleaseDna EndsRestriction DigestionRecombinant DnaDna ProcessingCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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