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What is common between linker and adapter in restriction digestion work?

Answer and explanation

Correct answer: Both can provide useful DNA ends for cloning

The governing concept is the use of short synthetic DNA molecules to modify or supply termini for cloning. Linkers and adapters are both designed nucleic-acid pieces that can be ligated to DNA fragments and can introduce a useful restriction site or a desired end configuration. Their exact structures and use are not identical: an adapter may provide compatible ends directly, while a linker often contains a restriction site that can be revealed by cleavage. Nevertheless, option A correctly states their common practical feature. They are not proteins, gel stains, or restriction enzymes. Enzymes catalyse reactions, whereas linkers and adapters are DNA substrates or structural components used in the cloning workflow.

Related tags

LinkerAdapterDna EndsCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq

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