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Subjects
Class 11BiotechnologyProcesses of Recombinant DNA TechnologyCutting of DNAEasy
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What is one way to make a blunt-ended PCR product cloning-ready in restriction digestion work?

Answer and explanation

Correct answer: Using adapter linker or primers with restriction site

Adapters linkers or engineered primers can provide desired ends. Strategy depends on cloning goal.

Related tags

Blunt Pcr ProductAdapterRestriction Site PrimerCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq
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10 more MCQs
  1. 1What are blunt ends?
  2. 2Where do restriction enzymes make cuts in DNA?
  3. 3What must be done before inserting a desired gene into a cloning vector?
  4. 4Which term best describes the action of a restriction enzyme?
  5. 5When are sticky ends in restriction-cut DNA useful?
  6. 6What can be done to a foreign gene before inserting it into a vector in cloning?
  7. 7Why are restriction-cut DNA fragments shown in gel electrophoresis?
  8. 8If plasmid and insert are cut with two different enzymes what can be a benefit?
  9. 9What problem may occur if the vector and insert have incompatible ends?
  10. 10Which enzymes are mainly used for cutting DNA?
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