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Why can restriction enzyme cutting at the end of PCR product be weak?Restriction enzyme से PCR product के end पर cut कमजोर क्यों हो सकता है?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: Enzyme may need extra bases outside the endenzyme को end के बाहर extra bases चाहिए हो सकते हैं
Some enzymes do not cut terminal sites efficiently. Adding extra bases in primer design is helpful.कुछ enzymes terminal sites पर efficient cut नहीं करते। Primer design में extra bases जोड़ना helpful है।
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