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What problem can occur if reaction tubes float during restriction digestion?

Answer and explanation

Correct answer: Temperature exposure can be uneven

If a reaction tube floats in a water bath, all parts of it may not receive the required temperature uniformly. This can reduce or make restriction-enzyme activity uneven, leading to incomplete DNA digestion. The enzyme does not become immediately inactive; the main issue is improper incubation temperature. Exam tip: Link efficient restriction digestion with the correct, uniform incubation temperature.

Related tags

Restriction DigestionRestriction EnzymesIncubation TemperatureDna CuttingRecombinant Dna TechnologyCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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