What is a way to avoid thawing the wrong enzyme in restriction digestion?

Correct answer: Verify the reaction plan and enzyme tube labels first

To select the correct enzyme, compare the enzyme named in the reaction plan with the label on the tube before thawing it. Restriction enzyme names may look similar, but their recognition sites can differ; a wrong enzyme may cut DNA at an unintended site. Thawing all enzymes is also poor practice because repeated thawing can reduce enzyme activity. Exam tip: check the enzyme name, buffer, and required incubation temperature before starting the reaction.