What should be one item in a restriction digestion checklist?

Correct answer: Enzyme name and buffer compatibility

Restriction digestion requires the correct restriction enzyme and a compatible buffer recommended for that enzyme. The buffer’s pH, salts, and cofactors affect enzyme activity; an unsuitable buffer may cause poor or no DNA cutting. Water taste, bench colour, and student height do not affect the reaction. Exam tip: check the enzyme, buffer, DNA, reaction volume, and incubation temperature before setting up digestion.