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What should be changed while repeating a failed restriction digestion?

Answer and explanation

Correct answer: Suspected variable, such as the correct buffer, enzyme amount, or DNA purity

When repeating a failed restriction digestion, check and modify the suspected variable, such as the appropriate buffer for the enzyme, the amount of active enzyme, or DNA purity. Changing one factor at a time helps identify the actual cause of failure. Changing all conditions together makes troubleshooting difficult. Exam tip: a restriction enzyme requires the correct buffer and sufficiently pure DNA for efficient digestion.

Related tags

Restriction DigestionRecombinant Dna TechnologyDna CuttingTroubleshootingRestriction EnzymesDna PurityCutting Of DnaProcesses Of Recombinant Dna Technology

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