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What effect can remaining salts in restriction digestion product have on ligation?Restriction digestion product में salts बचने से ligation पर क्या असर हो सकता है?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: Ligation efficiency can decreaseligation efficiency घट सकती है
Excess salts can affect ligase and DNA end interaction. Purification and wash step are necessary.Excess salts ligase और DNA end interaction को affect कर सकते हैं। Purification और wash step जरूरी हैं।
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