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Class 11BiotechnologyProcesses of Recombinant DNA TechnologyCutting of DNAEasy
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Why is purified restriction fragment quantified before ligation?

Answer and explanation

Correct answer: To keep insert-vector ratio correct

Fragment amount and molar ratio are important in ligation setup. Quantification improves cloning efficiency.

Related tags

Fragment QuantificationInsert Vector RatioLigationCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq
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10 more MCQs
  1. 1What are blunt ends?
  2. 2Where do restriction enzymes make cuts in DNA?
  3. 3What must be done before inserting a desired gene into a cloning vector?
  4. 4Which term best describes the action of a restriction enzyme?
  5. 5When are sticky ends in restriction-cut DNA useful?
  6. 6What can be done to a foreign gene before inserting it into a vector in cloning?
  7. 7Why are restriction-cut DNA fragments shown in gel electrophoresis?
  8. 8If plasmid and insert are cut with two different enzymes what can be a benefit?
  9. 9What problem may occur if the vector and insert have incompatible ends?
  10. 10Which enzymes are mainly used for cutting DNA?
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