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Why is UV exposure minimized during restriction fragment recovery?

Answer and explanation

Correct answer: To reduce DNA damage

UV light can damage DNA by causing lesions such as pyrimidine dimers and strand breaks. This may reduce the ability of the recovered restriction fragment to undergo ligation or cloning. Therefore, the DNA band should be viewed under UV for the shortest possible time. Exam tip: during gel-based DNA recovery, prolonged UV exposure is avoided because it damages DNA.

Related tags

Restriction Fragment RecoveryUv ExposureDna DamageGel ElectrophoresisDna CloningCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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