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When can cleanup after the first enzyme be necessary in sequential digestion?

Answer and explanation

Correct answer: When second buffer is incompatible with first

Salts or enzyme from first reaction can affect the second reaction. Cleanup or buffer exchange can help.

Related tags

Sequential DigestionCleanupBuffer ExchangeCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq

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