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Why is it necessary to mix buffer well in restriction digestion?

Answer and explanation

Correct answer: To keep salt and cofactors at uniform concentration

If buffer components are uneven enzyme activity can be affected. Gentle mixing increases reaction reliability.

Related tags

Buffer MixingCofactorsSalt ConcentrationCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq

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