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Subjects
Class 11BiotechnologyProcesses of Recombinant DNA TechnologyCutting of DNAEasy
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Why should the tip not be inserted repeatedly into enzyme stock during restriction digestion?

Answer and explanation

Correct answer: To reduce contamination risk

Repeated entry increases risk of contamination and enzyme loss. Use fresh sterile tip and careful pipetting.

Related tags

Enzyme StockPipettingContamination RiskCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq
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10 more MCQs
  1. 1What are blunt ends?
  2. 2Where do restriction enzymes make cuts in DNA?
  3. 3What must be done before inserting a desired gene into a cloning vector?
  4. 4Which term best describes the action of a restriction enzyme?
  5. 5When are sticky ends in restriction-cut DNA useful?
  6. 6What can be done to a foreign gene before inserting it into a vector in cloning?
  7. 7Why are restriction-cut DNA fragments shown in gel electrophoresis?
  8. 8If plasmid and insert are cut with two different enzymes what can be a benefit?
  9. 9What problem may occur if the vector and insert have incompatible ends?
  10. 10Which enzymes are mainly used for cutting DNA?
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