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What precaution is needed while cropping a gel image in restriction digest?

Answer and explanation

Correct answer: Relevant lanes and ladder context should not be removed

The governing principle is scientific transparency: image editing may improve presentation, but it must not remove information needed to interpret the experiment. When cropping a gel photograph, the relevant sample lanes, lane order, molecular-size ladder, and identifying labels or boundaries should remain visible whenever they are needed to understand the result. Removing the ladder can prevent size estimation, while removing lanes can hide controls or unexpected outcomes. Cropping must never involve inventing bands, hiding lane order, or changing contrast in a way that alters the apparent data. A clearly disclosed, non-misleading crop preserves both readability and evidential context, so option A is the correct precaution.

Related tags

Gel ImageCroppingScientific ReportingCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq

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