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Why should a lane legend be written with image record in restriction digestion?

Answer and explanation

Correct answer: To show what is in each lane

A lane legend is a documentation record that identifies the material loaded in each lane of a gel image. It may specify the DNA ladder, untreated control, restriction digest, different samples, and relevant experimental conditions. This information allows another person to connect each visible band with the correct sample and judge whether the observed pattern supports the expected digestion. Without a legend, lane positions can be forgotten or confused, so even a technically good image becomes difficult to interpret or reproduce. A legend does not chemically modify the enzyme or DNA, dry the gel, or remove DNA. Thus option A is correct because it preserves the meaning and traceability of the image.

Related tags

Lane LegendGel ImageDocumentationCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq

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