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What is the purpose of the dense component in loading dye?

Answer and explanation

Correct answer: Helping the sample settle into the gel well

Loading dye contains a dense component such as glycerol or sucrose. It increases the density of the sample so that the sample settles at the bottom of the gel well instead of dispersing into the buffer. DNA is cut by restriction endonucleases, not by loading dye. Exam tip: the coloured tracking dyes indicate the progress of electrophoresis; they do not show the position of DNA itself.

Related tags

Loading DyeAgarose Gel ElectrophoresisGlycerolDna Sample LoadingRecombinant Dna TechnologyCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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