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What can happen if sample wells are damaged in restriction digest gel?

Answer and explanation

Correct answer: The sample may leak or may not load properly

A damaged sample well may not retain the sample at the start of the gel. The sample can leak out, load unevenly, or spread into a nearby lane, producing faint, smeared, or irregular bands. Damage to a well does not change the restriction enzyme’s recognition or cutting sites. Exam tip: associate damaged wells mainly with faulty loading and poor band patterns.

Related tags

Restriction DigestAgarose Gel ElectrophoresisSample WellsDna LoadingBand PatternCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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