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What problem occurs due to lane overloading in restriction digestion?

Answer and explanation

Correct answer: Bands can appear diffuse or smeared

When too much DNA is loaded into one gel lane, the DNA fragments do not separate cleanly and the bands may appear broad, diffuse, or smeared. Complete absence of DNA usually indicates a sample or loading failure, not the direct effect of lane overloading. Exam tip: Load the recommended amount of DNA per lane to obtain sharp, well-resolved bands.

Related tags

Restriction DigestionGel ElectrophoresisLane OverloadingDna SmearingBand ResolutionCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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