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What problem arises from overexposure of gel image in restriction digestion?

Answer and explanation

Correct answer: Bands become saturated, making intensity comparison unreliable

With excessive exposure, very bright DNA bands can reach the detector's limit and appear saturated. Their actual intensities can then no longer be compared quantitatively and reliably. Image exposure does not change enzyme activity, DNA fragment size, or migration through the gel. Exam tip: associate overexposure mainly with poor image quality and unreliable band quantification.

Related tags

Restriction DigestionGel ElectrophoresisGel ImagingBand IntensityImage SaturationDna AnalysisCutting Of DnaProcesses Of Recombinant Dna Technology

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