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What is the general relation between cut sites and fragments in circular DNA?

Answer and explanation

Correct answer: Fragments generally equal cut sites

The governing concept is the relationship between restriction cuts and DNA fragments. For a circular DNA molecule, each distinct cut site usually opens the circle at one position. If there is one cut, the circular molecule is opened and becomes one linear fragment, not zero fragments. If there are two different cut sites, the circle is divided into two fragments; similarly, three cuts generally produce three fragments, provided the sites are distinct and the digestion is complete. Therefore, for a circular molecule, the usual fragment number equals the number of effective cut sites. The enzyme name alone does not determine the count; its recognition sites in that DNA sequence do.

Related tags

Circular DnaCut SitesFragment CountCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq

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