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In which situation can a frequent-cutter enzyme be useful?

Answer and explanation

Correct answer: To obtain small fragments for restriction pattern analysis

Direct answer: Option A, a frequent cutter can be useful for restriction-pattern analysis when many smaller fragments are wanted. A frequent-cutter enzyme recognizes a short or commonly occurring sequence, so it may cut a DNA molecule at many positions. This produces several relatively small fragments whose sizes can be separated on a gel and compared for mapping or analytical purposes. The exact usefulness depends on the DNA sequence and the experimental aim; too many fragments may also complicate interpretation. Option A is correct. Option B is wrong because cutting DNA does not convert it into protein. Option C is wrong because an enzyme is stored under suitable conditions, not by using it as a storage method. Option D is wrong because electrophoresis needs an appropriate buffer. Memory cue: frequent cutter means frequent sites and often smaller pieces.

Related tags

Frequent CutterSmall FragmentsRestriction Analysis

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