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How does a six-base restriction site generally differ from a four-base site?

Answer and explanation

Correct answer: It is less likely to occur in DNA

A six-base recognition sequence requires a particular arrangement of more bases than a four-base site. Therefore, in random DNA, a six-base restriction site generally occurs less frequently and the enzyme cuts at comparatively fewer locations. A four-base site occurs more often and can produce more fragments. Exam tip: the longer the recognition site, the lower its usual frequency of occurrence.

Related tags

Restriction EnzymesRestriction SiteDna CuttingRecombinant Dna TechnologyRecognition SequenceCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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