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What affects the frequency of a restriction site?

Answer and explanation

Correct answer: The length of the recognition sequence and DNA sequence composition

The shorter a restriction enzyme’s recognition sequence is, the more often it is generally expected to occur by chance in DNA. For example, in random DNA, a 4-base recognition site occurs more frequently than a 6-base site. Base composition, such as GC-rich or AT-rich DNA, also changes the frequency of a particular recognition sequence. The enzyme tube label, gel photograph, and pipette colour do not affect the presence of restriction sites in DNA. Exam tip: consider both recognition-sequence length and base composition when estimating site frequency.

Related tags

Restriction EnzymesRestriction SiteRecognition SequenceDna CompositionRecombinant Dna TechnologyCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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