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Why are Type II restriction enzymes preferred in cloning?

Answer and explanation

Correct answer: Because they give specific and predictable cuts

The direct answer is C: Type II enzymes give specific and predictable cuts. These enzymes recognize defined DNA sequences and cut at known positions, often producing sticky or blunt ends that can be planned for vector and insert preparation. This control makes orientation, joining, and checking of recombinant DNA much easier. Option A is wrong because random cutting would make cloning difficult and unpredictable. Option B is wrong because restriction enzymes do not make RNA. Option C is correct because defined recognition sites and reproducible cuts are their major cloning advantage. Option D is wrong because converting DNA information into protein requires transcription and translation, not restriction digestion. Exam cue: Type II enzymes are preferred because they cut where the experimenter can predict.

Related tags

Type Ii Restriction EnzymeSpecific CutVector Insert

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