What should be considered while choosing elution buffer after purification in restriction digestion?Restriction digestion में purification के बाद elution buffer choose करते समय क्या ध्यान रखें?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: Compatibility with next ligation or enzyme reactionnext ligation या enzyme reaction compatibility
Elution buffer should not contain components that inhibit ligation. Choose buffer according to downstream use.Elution buffer में ऐसे components न हों जो ligation inhibit करें। Downstream use के अनुसार buffer चुनें।
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