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Why is leftover restriction enzyme a problem in ligation setup after restriction digestion?Restriction digestion के बाद ligation setup में leftover restriction enzyme क्यों समस्या है?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: It can cut DNA againवह DNA को फिर काट सकता है
Active restriction enzyme can cut newly joined DNA in ligation mix. Cleanup or inactivation is useful.Active restriction enzyme ligation mix में newly joined DNA को काट सकता है। Cleanup या inactivation useful है।
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