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Subjects
Class 11BiotechnologyProcesses of Recombinant DNA TechnologyCutting of DNAEasy
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If ends are compatible but ligation fails in restriction digestion work what can be a cause?

Answer and explanation

Correct answer: Contaminants or wrong DNA ratio

Besides compatible ends clean DNA and correct ratio are necessary. Salt ethanol or enzyme carryover can block ligation.

Related tags

Ligation FailureContaminantsDna RatioCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnologyClass 11 Mcq
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10 more MCQs
  1. 1What are blunt ends?
  2. 2Where do restriction enzymes make cuts in DNA?
  3. 3What must be done before inserting a desired gene into a cloning vector?
  4. 4Which term best describes the action of a restriction enzyme?
  5. 5When are sticky ends in restriction-cut DNA useful?
  6. 6What can be done to a foreign gene before inserting it into a vector in cloning?
  7. 7Why are restriction-cut DNA fragments shown in gel electrophoresis?
  8. 8If plasmid and insert are cut with two different enzymes what can be a benefit?
  9. 9What problem may occur if the vector and insert have incompatible ends?
  10. 10Which enzymes are mainly used for cutting DNA?
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