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What is the benefit of briefly centrifuging restriction digest mix?

Answer and explanation

Correct answer: Components collect at bottom for proper reaction

Direct answer: Option A, a brief centrifuge spin collects droplets at the tube bottom and helps make the reaction uniform. Small drops can remain on the tube wall or lid after pipetting. A short spin brings them together, so enzyme, DNA, buffer, and water are in the same reaction volume and can mix during incubation. Option A is correct, although spinning alone does not replace careful pipetting or mixing. Option B is wrong: a brief spin does not intentionally inactivate the enzyme; excessive heat or unsuitable conditions could do that, not the normal quick spin. Option C is wrong because DNA does not become protein by centrifugation. Option D is wrong because a brief spin does not evaporate buffer. Use a suitable centrifuge and follow laboratory instructions. Memory cue: quick spin gathers, it does not change the chemistry.

Related tags

Brief CentrifugeReaction MixConsistent Incubation

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