Why should the tube cap be kept tightly closed during restriction-digest incubation?Restriction-digest incubation के दौरान tube का cap कसकर बंद क्यों रखना चाहिए?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: To reduce evaporation and maintain the intended reaction conditionsTo reduce evaporation and maintain the intended reaction conditions
Restriction digestion is performed in a small reaction volume with defined concentrations of DNA, enzyme, salts, magnesium, and buffer. During incubation, especially at an elevated temperature, evaporation can reduce the volume and concentrate the remaining components. This changes the intended salt, substrate, and enzyme concentrations and may produce less reproducible digestion or even inhibit the enzyme. A properly closed tube helps retain water and keeps the reaction close to its designed composition. Therefore option A is correct. The cap does not stain DNA, destroy the restriction enzyme, or freeze agarose. Good practice also includes briefly spinning down the mixture and following the recommended incubation temperature and time.Restriction digestion छोटे reaction volume में किया जाता है, जिसमें DNA, enzyme, salts, magnesium और buffer की निश्चित concentrations होती हैं। Incubation के दौरान, विशेषकर ऊंचे तापमान पर, evaporation से volume कम हो सकता है और बचे हुए components अधिक concentrated हो सकते हैं। इससे salt, substrate और enzyme की वास्तविक concentrations बदल जाती हैं तथा digestion कम reproducible हो सकता है या enzyme की activity प्रभावित हो सकती है। Tube को ठीक से बंद रखने से पानी का नुकसान घटता है और reaction अपनी निर्धारित composition के निकट रहता है। इसलिए option A सही है। Cap DNA को stain नहीं करता, restriction enzyme को digest नहीं करता और agarose gel को freeze नहीं करता; B, C और D असंगत हैं।
Student feedback
Was this question useful?
👍 0 Helpful 👎 0 Not helpful
Yes 0% No 0%
0 responsesStudent Reviews
No published reviews yet.
