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When is DNA cleanup helpful before restriction digestion?

Answer and explanation

Correct answer: When the sample contains contaminants such as salts, phenol, or ethanol

Contaminants such as salts, phenol, and ethanol can reduce or inhibit restriction enzyme activity and may interfere with the required buffer conditions. Therefore, cleaning a contaminated DNA sample makes restriction digestion more efficient and reliable. In option B, the DNA is already pure, so additional cleanup is usually unnecessary. Exam tip: remove contaminants before enzyme-based molecular biology procedures.

Related tags

Restriction DigestionDna CleanupDna ContaminantsRestriction EnzymesRecombinant Dna TechnologyCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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