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Which step helps reduce star activity?

Answer and explanation

Correct answer: Using the recommended buffer and a limited amount of enzyme

In star activity, a restriction enzyme may cut DNA sequences that resemble, but are not its normal recognition sites. Using the recommended buffer, correct pH, and a limited amount of enzyme maintains enzyme specificity; therefore, option A is correct. Excess enzyme, prolonged incubation, or an inappropriate pH can increase star activity. Exam tip: in restriction digestion, follow the recommended buffer, enzyme amount, and incubation time.

Related tags

Star ActivityRestriction EnzymesDna DigestionRecombinant Dna TechnologyRestriction EndonucleaseEnzyme SpecificityCutting Of DnaProcesses Of Recombinant Dna Technology

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