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How can complete digestion be confirmed?

Answer and explanation

Correct answer: By observing the expected fragment bands and absence of an uncut DNA band on agarose gel electrophoresis

In complete digestion, the restriction enzyme cuts DNA at all of its target sites. Therefore, agarose gel electrophoresis shows bands of the expected DNA fragments, with no band for the original uncut DNA. Seeing only an uncut band indicates that digestion has not occurred or is incomplete. Exam tip: confirm digestion by comparing the observed band pattern with the expected fragment sizes.

Related tags

Restriction DigestionAgarose Gel ElectrophoresisDna FragmentsUncut DnaRecombinant Dna TechnologyCutting Of DnaProcesses Of Recombinant Dna TechnologyBiotechnology

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